Coding

Part:BBa_K1321159

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)

Fmt metallothionein with T7 promoter

Fmt is a metallotionein (metal binding peptide) from the seaweed Fucus vesiculosus and was first submitted by the Groningen 2009 iGEM team as BBa_K190019 (please refer to this page for detailed information on characteristion). Our version of the part has been made compatible with RFC25 fusion format at codon optimised for E. coli and is driven by the T7 promoter.

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None